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Gibco/12100046/高糖DMEM干粉/現(xiàn)貨

  • 發(fā)布日期:2017-12-29      瀏覽次數(shù):2164
    • 12100046DMEM, powder, high glucose

      Gibco/12100046/高糖DMEM干粉/現(xiàn)貨

      訂貨找螞蟻淘就對(duì)了,4000-520-626

      描述

      DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
      This DMEM is modified as follows:

      WithWithout
      • High Glucose• Sodium Pyruvate
      • L-glutamine• HEPES
      • Phenol Red• Sodium Bicarbonate


      The complete formulation is available.

      Using DMEM
      DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

      cGMP manufacturing and quality system
      DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

      For In Vitro Diagnostic Use.

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